Integrated Assessment of Heavy Metal Contamination in Sediments from a Coastal Industrial Basin, NE China

The purpose of this study is to investigate the current status of metal pollution of the sediments from urban-stream, estuary and Jinzhou Bay of the coastal industrial city, NE China. Forty surface sediment samples from river, estuary and bay and one sediment core from Jinzhou bay were collected and analyzed for heavy metal concentrations of Cu, Zn, Pb, Cd, Ni and Mn. The data reveals that there was a remarkable change in the contents of heavy metals among the sampling sediments, and all the mean values of heavy metal concentration were higher than the national guideline values of marine sediment quality of China (GB 18668-2002). This is one of the most polluted of the world’s impacted coastal systems. Both the correlation analyses and geostatistical analyses showed that Cu, Zn, Pb and Cd have a very similar spatial pattern and come from the industrial activities, and the concentration of Mn mainly caused by natural factors. The estuary is the most polluted area with extremely high potential ecological risk; however the contamination decreased with distance seaward of the river estuary. This study clearly highlights the urgent need to make great efforts to control the industrial emission and the exceptionally severe heavy metal pollution in the coastal area, and the immediate measures should be carried out to minimize the rate of contamination, and extent of future pollution problems

Elective cancer surgery in COVID-19-free surgical pathways during the SARS-CoV-2 pandemic: An international, multicenter, comparative cohort study

PURPOSE As cancer surgery restarts after the first COVID-19 wave, health care providers urgently require data to determine where elective surgery is best performed. This study aimed to determine whether COVID-19–free surgical pathways were associated with lower postoperative pulmonary complication rates compared with hospitals with no defined pathway. PATIENTS AND METHODS This international, multicenter cohort study included patients who underwent elective surgery for 10 solid cancer types without preoperative suspicion of SARS-CoV-2. Participating hospitals included patients from local emergence of SARS-CoV-2 until April 19, 2020. At the time of surgery, hospitals were defined as having a COVID-19–free surgical pathway (complete segregation of the operating theater, critical care, and inpatient ward areas) or no defined pathway (incomplete or no segregation, areas shared with patients with COVID-19). The primary outcome was 30-day postoperative pulmonary complications (pneumonia, acute respiratory distress syndrome, unexpected ventilation). RESULTS Of 9,171 patients from 447 hospitals in 55 countries, 2,481 were operated on in COVID-19–free surgical pathways. Patients who underwent surgery within COVID-19–free surgical pathways were younger with fewer comorbidities than those in hospitals with no defined pathway but with similar proportions of major surgery. After adjustment, pulmonary complication rates were lower with COVID-19–free surgical pathways (2.2% v 4.9%; adjusted odds ratio [aOR], 0.62; 95% CI, 0.44 to 0.86). This was consistent in sensitivity analyses for low-risk patients (American Society of Anesthesiologists grade 1/2), propensity score–matched models, and patients with negative SARS-CoV-2 preoperative tests. The postoperative SARS-CoV-2 infection rate was also lower in COVID-19–free surgical pathways (2.1% v 3.6%; aOR, 0.53; 95% CI, 0.36 to 0.76). CONCLUSION Within available resources, dedicated COVID-19–free surgical pathways should be established to provide safe elective cancer surgery during current and before future SARS-CoV-2 outbreaks

Elective Cancer Surgery in COVID-19-Free Surgical Pathways During the SARS-CoV-2 Pandemic: An International, Multicenter, Comparative Cohort Study.

PURPOSE: As cancer surgery restarts after the first COVID-19 wave, health care providers urgently require data to determine where elective surgery is best performed. This study aimed to determine whether COVID-19-free surgical pathways were associated with lower postoperative pulmonary complication rates compared with hospitals with no defined pathway. PATIENTS AND METHODS: This international, multicenter cohort study included patients who underwent elective surgery for 10 solid cancer types without preoperative suspicion of SARS-CoV-2. Participating hospitals included patients from local emergence of SARS-CoV-2 until April 19, 2020. At the time of surgery, hospitals were defined as having a COVID-19-free surgical pathway (complete segregation of the operating theater, critical care, and inpatient ward areas) or no defined pathway (incomplete or no segregation, areas shared with patients with COVID-19). The primary outcome was 30-day postoperative pulmonary complications (pneumonia, acute respiratory distress syndrome, unexpected ventilation). RESULTS: Of 9,171 patients from 447 hospitals in 55 countries, 2,481 were operated on in COVID-19-free surgical pathways. Patients who underwent surgery within COVID-19-free surgical pathways were younger with fewer comorbidities than those in hospitals with no defined pathway but with similar proportions of major surgery. After adjustment, pulmonary complication rates were lower with COVID-19-free surgical pathways (2.2% v 4.9%; adjusted odds ratio [aOR], 0.62; 95% CI, 0.44 to 0.86). This was consistent in sensitivity analyses for low-risk patients (American Society of Anesthesiologists grade 1/2), propensity score-matched models, and patients with negative SARS-CoV-2 preoperative tests. The postoperative SARS-CoV-2 infection rate was also lower in COVID-19-free surgical pathways (2.1% v 3.6%; aOR, 0.53; 95% CI, 0.36 to 0.76). CONCLUSION: Within available resources, dedicated COVID-19-free surgical pathways should be established to provide safe elective cancer surgery during current and before future SARS-CoV-2 outbreaks

Atmospheric sources of trace element contamination in cultivated urban areas: A review

Producing food in cities has garnered increasing attention over the past decade. Although there are ecological and social benefits, cultivated urban areas (CUAs) also bear contamination hazards, including from trace elements (TEs). Trace element contamination has been studied extensively in CUAs, but atmospheric sources remain understudied and poorly understood. A brief discussion is offered on atmospheric particulate deposition processes in cities and their implications for urban food production. Available findings are discussed and contrasted. Existing research assesses atmospheric deposition indirectly or otherwise lacks controls for other TE contaminants. There is little to no engagement with methodological guidelines from the atmospheric sciences, which reduces confidence in the findings so far attained. Suggestions are delineated to combine techniques used in the atmospheric sciences with the robust methodologies already generated by studies on TE contamination in CUAs, such as isotope and TE ratios analyses

Problemy związane z jakością wód powierzchniowych wykorzystywanych do nawodnień w delcie rzeki Neretwa (Chorwacja)

The research was set up in the Neretva River valley in the Southern part of Croatian Karst area, where implementation of modern hydrotechnical practices within the river catchment's area led to intrusion of seawater to groundwater resulting in soil salinization in the delta. The region has great agro-ecological potential for intensive production of vegetables and Mediterranean fruits. Since the combination of the effects of saline groundwater and the use of this water for irrigation may have disastrous effects on the productivity of agricultural soils water, a project was started in order to set up a permanent monitoring network. The aim of this study was to determine the salt dynamics in the surface water on five locations which are considered as potential sources of the irrigation water (Modric canal, Neretva River near Opuzen, Crepina, Jasenska and Vidrice pumping station) during a 4-year period (1999-2002). The surface water samples had been collected on monthly basis and analyzed for all parameters required in the irrigation water quality classification. The results show considerable spatial and temporal variability of determined parameters. Thus, in the Neretva River near Opuzen, total salt concentrations in water ranged from 0.4 to 7.7 dS·m-¹, and in Modric from 1.65 up to17.2 dS·m-¹. Dominant cations and anions on all observed locations were Na+ and Cl-. Constantly high concentration of Na+ in sampled surface waters is of a special concern. Utilization of the water of such quality may cause problems related to the use of alkaline waters for irrigation, which can further cause permanent loss of fertile soil.Badania prowadzono w dolinie Neretwy, w południowej części chorwackiego obszaru krasowego, na którym działania hydrotechniczne w zlewni doprowadziły do napływu wody morskiej do warstw wodonośnych i w konsekwencji spowodowały zasolenie gleb w delcie rzeki. Region charakteryzuje się dużym potencjałem agroekologicznym i możliwością produkcji warzyw i owoców śródziemnomorskich. Ponieważ nałożenie się efektów zasolenia wód gruntowych i użycia tych wód do nawodnień może mieć fatalne skutki dla produktywności gleb, zainicjowano założenie sieci stałego monitoringu jakości wód. Celem tych badań było określenie dynamiki zasolenia wód powierzchniowych w czteroletnim okresie (1999-2002) na podstawie pomiarów w pięciu stanowiskach uznanych za potencjalne źródła wody do nawodnień (Kanał Modric, Neretwa koło Opuzen oraz stacje pomp Crepina, Jasenka i Vidrice). Próbki wód powierzchniowych pobierano co miesiąc i analizowano w nich wszystkie parametry niezbędne do oceny jakości wód do nawodnień. Stwierdzono znaczną zmienność mierzonych parametrów w czasie i w przestrzeni. Całkowita mineralizacja wód Neretwy koło Opuzen wynosiła od 0,4 do 7,7 dS·m-¹ a w Kanale Modric od 1,65 do 17,2 dS·m-¹. Na+ i Cl- były dominującymi jonami we wszystkich badanych stanowiskach. Stale duże stężenie Na+ w próbkach wód powierzchniowych wymaga szczególnej troski. Stosowanie wody takiej jakości może powodować problemy związane z użyciem do nawodnień wód alkalicznych, co w przyszłości może prowadzić do całkowitej utraty żyznej gleby

A simple PCR with different 3 ends of the third primer for detection of defined point mutations: HCV genotyping as an example

In this article, we describe a useful modification of the polymerase chain reaction for amplification applicable to hepatitis C virus genotyping and determination of its subtypes. The method is fast, cheap and simple for detection of any known point mutation, and could be used in every laboratory with experience in polymerase chain reaction technique. We could differentiate hepatitis C virus subtype Ib from other subtypes and 2b from 2a and other subtypes as well. We could also differentiate hepatitis C type 3 using a type-specific oligonucleotide from 3a subtype, thus covering the most common hepatitis C virus (sub)types present in the European region.1st IFCC Roche Conference on Recent Progress in Molecular biology Technology, Mar 15-18, 1998, Singapore, Singapor

Hepatitis C virus RNA testing by nested PCR in blood preparations in Yugoslavia

Patients receiving any kind of human blood preparations are in permanent danger of any infection including hepatitis C (HCV) infection. Testing for the presence of HCV in blood preparations is one of the steps towards safe medical treatment, One of the approaches for this testing is a detection of HCV nucleic acid. In this paper we describe a simple method for isolation of HCV RNA from blood preparations and control of HCV RNA presence in 19 intravenous and intramuscular products, manufactured in the National Blood Transfusion Institute in Belgrade. RT-PCR was performed according the rules saving RNA. Primers were located in 5 conserved region. Seven out of 19 batches of gamma-globulin, albumin, anti-tetanus and anti-rabies immunoglobulin preparations were found to be HCV RNA positive. For the time being, the PCR method is too expensive for routine HCV RNA testing of hundreds of;blood donors per day. Serological screening test of blood donors and nested PCR testing for HCV RNA. in blood preparations could be an efficient combination of tests in prevention of posttransfusion hepatitis C. (C) 1998 Elsevier Science Ltd. All rights reserved